You can view barcode reports for sequencing runs that use barcode adapters during library preparation for samples. The reports show key performance metrics for each barcode included in the run.
The number of barcodes in the barcode report reflects the barcode set that was used in the run and the barcodes that are present in the sample. Data is included only for barcodes that are present in the run.
In the Data tab, click Completed Runs & Reports.
In the list of runs, find the run of interest, then click the link in the Report Name column.
In the left navigation menu, click Output Files, then scroll to the barcode section of the run report.
The barcode section of a run report displays the following information and provides access to downloadable files for each barcode:
Column
Description
Barcode Name
The individual barcode in the barcode set.
The row labeled No barcode reports on unclassified barcodes, which are reads that could not be classified as a match for one of the expected barcodes in the barcode set.
Sample
Name of the sample that was sequenced on instrument.
Bases
Post-filtering base output for each barcode.
% ≥ Q20
The percentage of reads that have a predicted quality score of Q20 or better.
Reads
Total number of filtered and trimmed library reads (independent of length). This number is reported in the barcode BAM file.
Mean Read Length
The average read length, in base pairs (bp), of all filtered and trimmed library reads reported in the BAM file for the barcoded run.
Read Length Histogram
A thumbnail histogram of the read lengths for this barcode. Click the thumbnail histogram to open a larger image.
UBAM
Download a binary file that contains unaligned or unmapped reads. Viewing the file requires a viewer application, such as the Integrative Genomics Viewer from the Broad Institute.
BAM
Download a compressed, binary form of the SAM file. The BAM file contains aligned reads sorted by genome reference location.
BAI
Download the BAM index (BAI) file. This file speeds up the access time for a coordinate-sorted BAM file.
