The 6-column target regions BED file format is used when some or all of the gene names are
known. BED files that are generated by AmpliSeq.com use this 6-column format.
The track line is required in a 6-column target regions BED file. The track line includes the
following information.
Field
Type
Description
name
(Optional)
String
A unique design identifier.
description
(Optional)
String
A description of the design.
type
(Required)
String
Must be bedDetail (without quotes).
ionVersion
String
Version of Torrent Suite™ Software.
The following is an example track line:
track name="ASD270245" description="AmpliSeq Pool ASD270245" type=bedDetail
In a 6-column target regions BED file, the coordinates lines require the following fields.
Field
Type
Description
chrom
String (chars ≥ 0x20, other than \tab)
The name of the chromosome. This name must be an exact match with a chromosome in the reference.
chromStart
Unsigned int64
The starting position of the feature (zero-based).
chromEnd
Unsigned int64
The ending position of the feature (not inclusive). Must be greater than chromStart.
AmpliconID
String
Amplicon ID. If missing, the following string is used "chrom" + ":" + "chromStart" + "-" + "chromEnd"
ID
String
A customer-specified ID. If missing, set to '.'. This field is not used.
GeneSymbol
String
The gene name. If missing, set to '.'.
Partial example of a 6-column target regions BED file:
