The track line is required in a hotspots BED file.
The following is an example track line:
track name="ASD270245" description="HotSpots locations for AmpliSeq ASD270245" type=bedDetail db=hg38 reference=GRCh38.p2
The track line includes these tab-separated fields:
Field
Type
Description
track name
(Optional)
String
A unique design identifier.
description
(Optional)
String
A description of the design.
type
(Required)
String
Must be bedDetail (without quotes).
db
(Optional)
String
The UCSC Assembly identifier.
reference
(This field is optional for hg19, and is required for GRCh38.)
String
The Ion Torrent™ Server reference identifier.
In hotspots BED files, the coordinates lines require these tab-separated fields:
Field
Type
Description
chrom
String (chars >= 0x20, other than \tab)
The name of the chromosome. This name must be an exact match with a chromosome in the reference.
chromStart
Unsigned int64
The starting position of the feature (zero-based).
chromEnd
Unsigned int64
The ending position of the feature (not inclusive). Must be greater than chromStart.
HotSpotName
String
The name is either the COSMIC identifier, dbSNP identifier, or is user-defined. If missing, the following string is used "chrom" + ":" + "chromStart" + "-" + "chromEnd"
HotSpotAlleles
String
A string that describes the variant using the following format: REF=reference_allele ; OBS=observed_allele ; ANCHOR=base_before_allele
AmpliconID
String
The Amplicon identifier. If missing, the following string is used "chrom" + ":" + "chromStart" + "-" + "chromEnd"
